The following information was submitted:
Transactions: WSEAS TRANSACTIONS ON BIOLOGY AND BIOMEDICINE
Transactions ID Number: 52-319
Full Name: Satish Pingale
Position: Researcher
Age: ON
Sex: Male
Address: Analytical Chemistry Research Laboratory, Mithibai College Vile Parle (W), Mumbai 400056
Country: INDIA
Tel: +919867540009
Tel prefix:
Fax:
E-mail address: sgpingale_pk@yahoo.co.in
Other E-mails: sgpingale_pk@rediffmail.com
Title of the Paper: Determination of amoxicillin in human plasma by LC-MS/MS and its application to a bioequivalence study
Authors as they appear in the Paper: Satish Pingale, Madhukar Badgujar, Kiran Mangaonkar, Nikos Mastorakis
Email addresses of all the authors: sgpingale_pk@yahoo.co.in, kvmangaonkar@indiatimes.com
Number of paper pages: 13
Abstract: An analytical method based on solid phase extraction has been developed and validated for analysis of amoxicillin in human plasma using gemifloxacin as an internal standard. A COSMOSIL 5C18-PAQ column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves simple isocratic chromatographic conditions and mass spectrometric detection in the positive ionization mode using an API-3000 system. The proposed method has been validated with linear range of 100–15000 ng/mL for amoxicillin. The intra-run and inter-run precision values are within 3.53% and 5.63% respectively for amoxicillin at LOQ level. Total elution time was as low as 2 min. This validated method was used successfully for analysis of plasma samples from a bioequivalence study.
Keywords: Amoxicillin, Antibiotic, Automated solid phase extraction, Tandem mass spectrometry, Human plasma, Bioequivalence study
EXTENSION of the file: .doc
Special (Invited) Session:
Organizer of the Session:
How Did you learn about congress:
IP ADDRESS: 59.95.22.101
