The following information was submitted:
Transactions: WSEAS TRANSACTIONS ON BIOLOGY AND BIOMEDICINE
Transactions ID Number: 89-113
Full Name: Carla Falugi
Position: Professor
Age: ON
Sex: Female
Address: Vilae Benedetto XV, N° 5, Genova, I-16132
Country: ITALY
Tel: +39 010 353 8055
Tel prefix: +39 010
Fax: +39 010 353 8047
E-mail address: falugi@unige.it
Other E-mails: carlafalugi@hotmail.it
Title of the Paper: New aspects of the relationship between acetylcholinesterase activity and cancer. I: Poly-APS experiments
Authors as they appear in the Paper: Ana Zovko, Kristina Sepcic, Tom Turk, Marco Faimali, Francesca Garaventa, Elisabetta Chelossi, Laura Pleari, Carla Falugi, Maria Grazia Aluigi, Cristiano Angelini, Lorenzo Gallus, Sara Ferrando
Email addresses of all the authors: Krisrina.sepcic@bf.uni-lj.si,tom.turk@bf.uni-lj.si, marco.faimali@ismar.cnr.it,francesca.garaventa@ismar.cnr.it, laura.paleari@istge.it,falugi@unige.it,mariagraziaaluigi@libero.it,cristiano.angelini@unige.it,gallus@unige.it.sara.ferrando@unige.it
Number of paper pages: 12
Abstract: Abstract: - Since the first '80s, we found that some tumor types, and in particular lung tumors present increase, or generally change in acetylcholinesterase activity. Acetylcholinesterase is an enzyme associated to the cholinergic signal system, whose classic role is to remove acetylcholine from the receptors. Nevertheless, it is also involved in cell-to-cell communication driving embryonic development and in the regulation of several cellular features, such as apoptosis and cell movements. The presence of molecules related to the cholinergic signal system in the healthy and carcinogenic lung tissues, raised the hypothesis that substances inhibiting or affecting the cholinergic signaling system could exert an anti-cancer action at least in these tissues. Cytotoxicity tests on immortalized and primary cell lines derived from lung tumor (NSCLC) showed an AChE inhibition-dependent selective reduction of cell viability, statistically significant. The same cells, expos!
ed to non toxic AChE inhibitors exhibited a loss in the mitochondrial potential, characteristic of the early apoptotic events and showed positive response to the annexin V assay, and to the T-terminal assay, that are specific features of the apoptotic event. Moreover, three-dimensional cell cultures (spheroids) of tumor cells, on exposure to AChE inhibitors show a decrease in the membrane-linked oligosaccharides, that are responsible for the adhesivity of the metastatic cells. In this promising picture, the need emerges of further studies aimed at understanding the effects of AChE inhibition on the regulation of acetylcholine release and the effects of AChE inhibitors on the functioning of acetylcholine receptors.
Keywords: Anti-cancer,Natural drug,Lung cancer,Acetylcholinesterase,Apoptosis,Cell proliferation,Reniera sarai sponge.
EXTENSION of the file: .pdf
Special (Invited) Session: Studies on a promising anticancer molecule of marine origin.
Organizer of the Session: 618-522
How Did you learn about congress: zoltan Rakonczay, University of Szeged, email rz@comser.szote.u-szeged.hu; Luciana Dini, University of Lecce email laucina.dini@unile.it
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