The following information was submitted:
Transactions: WSEAS TRANSACTIONS ON BIOLOGY AND BIOMEDICINE
Transactions ID Number: 28-224
Full Name: Zaini Mohd-Zain
Position: Senior Lecturer
Age: ON
Sex: Female
Address: Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, 40450 Shah Alam, Selangor
Country: MALAYSIA
Tel: 55443974
Tel prefix: ++603
Fax: ++603 55442831
E-mail address: zainimz@salam.uitm.edu.my
Other E-mails: zainimz@yahoo.com
Title of the Paper: Phenotypic Characteristics, Antibiotic Susceptibility and Pathogenicity of Ornithobacterium rhinotracheale
Authors as they appear in the Paper: Zaini Mohd-Zain, Tan Lin Jee and Kamaruzaman Jusoff
Email addresses of all the authors: zainimz@salam.uitm.edu.my, tanlj@jphvri.gov.my, jusoff.kamaruzaman@yale.edu
Number of paper pages: 10
Abstract: Abstract:- Ornithobacterium rhinotracheale (ORT) has been recently recognized as one of the respiratory pathogen in poultry. This bacterium produces clinical signs in chickens which are almost similar to the infection of Avibacterium paragallinarum, thus making diagnosis difficult. A collection of 18 isolates of O. rhinotracheale obtained from diseased chickens in Malaysia were characterized and their pathogenicity in embryonated eggs and chickens was investigated and compared to a reference strain. Antimicrobial susceptibility pattern of these isolates against seven antibiotics were determined using Kirby-Bauer diffusion test while minimal inhibition concentration (MIC) of tylosin and tilmicosin were performed using agar dilution method following the guidelines of Clinical and Laboratory Standards Institute. Biochemical and enzymatic tests results showed that the O. rhinotracheale isolates were made up of two variants that differ from the reference strain in their!
fermentation of arginine and glucose. Serotyping of these isolates revealed that all of the isolates were serotype A. Antimicrobial susceptibility test performed using antimicrobial disks showed that at least two of the isolates were resistant to more than three of the antibiotics tested. High (>128 ƒÝg/ml) minimal inhibition concentration (MIC) of tylosin and tilmicosin was observed in the isolates. Two representatives from these isolates were tested for their ability to cause mortality in egg embryos and their clinical manifestations in specific-pathogen-free (SPF) chickens. When compared to uninoculated embryos, these two isolates were found to cause significantly (P<0.01) higher number of embryo mortality. Infection of these isolates in SPF chickens caused growth retardation but no respiratory symptoms were observed. The results obtained in this study have provided some basic information on the properties of O. rhinotracheale that would be useful in diagnosing the dise!
ase. The understanding of its role in poultry health provides some inf
ormation that could be useful in implementing preventive measures against the disease.
Keywords: Key-Words: - Ornithobacterium rhinotracheale, embryo mortality, growth retardation, chickens, antibiotic resistant, Malaysia
EXTENSION of the file: .doc
Special (Invited) Session: A Preliminary Investigation on the Phenotypic Characteristics of Ornithobacterium rhinotracheale
Organizer of the Session: 595-394
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