The following information was submitted:
Transactions: WSEAS TRANSACTIONS ON BIOLOGY AND BIOMEDICINE
Transactions ID Number: 53-316
Full Name: Dariusz Szukiewicz
Position: Associate Professor
Age: ON
Sex: Male
Address: Medical University of Warsaw, ul.Krakowskie Przedmiescie 26/28, 00-928 Warsaw
Country: POLAND
Tel: +48228268141
Tel prefix:
Fax: +48228264585
E-mail address: Dszukiewicz@hotmail.com
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Title of the Paper: Bradykinin B1 receptor overexpression is positively correlated with increased histidine decarboxylase (HDC) activity – a potential role in the pathophysiology of diabetic placenta
Authors as they appear in the Paper: Dariusz Szukiewicz, Michal Pyzlak, Habib Alkhalayla, Arlena Szukiewicz, Slawomir Maslinski
Email addresses of all the authors: Dszukiewicz@hotmail.com
Number of paper pages: 11
Abstract: Hiperhistaminaemia and increased bradykinin have been found in diabetic placentae. Both, histamine and bradykinin are well known mediators of inflammation. Bradykinin may stimulate mast cell degranulation, increasing the local histamine level. Bradykinin B1 receptor shows involvement in the inflammatory response, whereas the B2 receptor mediates most of the effects induced by kinins. In this study we examined comparatively (diabetes class C vs normal pregnancy) correlations between placental HDC activity, histamine concentration, and placental bradykinin receptors B1 and B2 expression. Sixteen diabetic placentae and 16 normal (control) placentae were collected (Group I and II, respectively). Activity of HDC was assayed in placental samples obtained in a standardized manner, using a modified method of Endo. Histamine concentrations in placental cuts were estimated fluorimetrically, whereas expression of B1 and B2 was examined in immunostained paraffin sections, usi!
ng quantitative morphometry in the areas matched in mean vascular density. Mean HDC activity in diabetic placenta was significantly (p<0.05) increased compared to controls (3.97 +/-0.25 vs 2.88 +/-0.15 nmol/h/g +/-SEM). Histamine concentration was also significantly increased in diabetes (387 +/-25.3 vs 239 +/-14.3 ng/g of wet weight +/-SEM; Group I and Group II, respectively). Mean expression of the B1 was augmented in diabetes and reached 289.8% of the value observed for Group II (p<0.05). Mean expression of B2 receptors was similar in both studied groups. Increased HDC activity may be responsible for some of the pathophysiologic events observed in diabetic materno-placento-fetal compartment. HDC-dependent histamine elevation may change locally vascular properties by influence on bradykinin receptors expression. Proinflammatory changes mediated via B1 should be expected rather, than modified vasomotor reactivity related to B2. Angiogenic properties of histamine and kinins!
may also be of great importance hin diabetic placental tissue.
Keywords: Histidine decarboxylase, Histamine, Bradykinin receptors, Human placenta, Diabetes class C, Pregnancy
EXTENSION of the file: .doc
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How Did you learn about congress: Cell Biology, Immunology, Pathophysiology, Quantitative morphometry, Embryology, Obstetrics
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